Traditional methods such as biochemical assays, manual microscopy, Western blotting and flow cytometry are all valuable techniques to the cell researcher, but are limited by several drawbacks:
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low throughput
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labor-intensive
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destructive
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relatively low information content
High content analysis employs high throughput, automated microscopy, facilitating cellular assays that enable scientists to ask questions that were not previously possible to address. Greater throughput, together with the crucial ability to measure multiple parameters simultaneously in the same cells, means that HCA assays are significantly more informative than traditional cell research techniques.