IN Cell Investigator level 2 analysis provides structured and guided method of image analysis where multi-parametric classification of cells into subpopulations is required. With increased flexibility in analysis approach and design compared to pre-configured modules, it is applicable to a wide range of assays that utilise fluorescent markers to measure a cellular process.
It is ideal for:
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Sub population studies
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Cell viability
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Cell cycle monitoring
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Apoptosis
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Cell signalling
This flexible analysis tool has been developed to guide analysis of complex multicolor cell based assays with user-defined classification strategies - interactive filters (decision trees) enable the definition of subpopulations.
Guided analysis enables you to:
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Optimize segmentation methods to identify cells, nuclei and organelles
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Group different measurements according to cellular components and to describe cellular phenotypes in detail
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Perform advanced subpopulation analysis through intuitive classification tools such as Decision Trees
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Use any combination of available intensity, morphological and neighborhood measurements to classify cells into relevant subpopulations e.g. to define cell cycle status or to exclude untransfected cells
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Filter using threshold, 2D linear discriminant and multi level decision trees
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Use interactive plots to provide instant feedback between images and data to aid optimal parameter setting
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Use multidimensional graphical displays to visualize characteristics of cells within a population
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Report a wide range of intensity and morphology based output measures
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Build complex hierarchical classification systems by linking multiple subpopulation analyses.
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Quantitating responses of subpopulations in cellular assays using the IN Cell Analyzer 1000 Multi Target Analysis Module (3 MB)
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