Level 1: Pre-configured analysis

IN Cell Investigator level 1 analysis provides preconfigured analysis modules suitable for the majority of assay types and have been validated for over 50 applications.

IN Cell Investigator pre-configured analysis modules are ideal for:

	•		New high content assay users, unfamiliar with image analysis of cells
	•		Multi-user environments with a need to maintain consistent analysis and to get started quickly
	•		Screening assays where throughput, reliability and reproducibility are paramount

IN Cell Investigator pre-configured analysis modules provide:

	•		Consistent ease of use through a wizard-style user interface
	•		Optimized segmentation methods to identify cells, nuclei and organelles
	•		Visible results through configurable heat maps, scatter and histogram plots

Micronuclei Analysis Module

IN Cell Investigator pre-configured analysis modules enable many assay types - including:

	•		Apoptosis
	•		Signaling pathway analysis
	•		Toxicity
	•		Cell cycle reporting
	•		Organelle and protein trafficking studies
	•		Neurite outgrowth
	•		Cell differentiation
	•		Tissue imaging
	•		Cell tracking

	•		Angiogenesis
	•		Receptor activation
	•		Calcium imaging
	•		Micronuclei formation
	•		Morphology analysis
	•		Neuronal function
	•		RNAi analysis
	•		Cell migration
	•		Cytoskeletal analysis

Modules included with IN Cell Investigator Software:

	•		Micronuclei formation: Fast and automated analysis of micronucleus formation for toxicological applications

	•		Neurite outgrowth: Measurement of neurites and cell morphology features

	•		Nuclear trafficking: Measures changes in the ratio of fluorescence intensity between the cytoplasm and the nucleus

	•		Granularity: Quantitates ‘granules' in terms of count, area, and intensity

	•		Morphology: Measures cellular morphology and cellular intensity of nuclear and cytoplasmic compartments

	•		Cell cycle trafficking: Assigns cells expressing an appropriate fluorescent label to G2, M, or G1/S phases

	•		Object analysis: Measures changes in the intensity of fluorescent labels

	•		Dual area object analysis: Enables simultaneous measurement of subcellular inclusions such as grains, vesicles, and fibers (e.g., for apoptosis analysis)

	•		Membrane trafficking: Measures subcellular inclusions and translocations of proteins to and from the plasma membrane

	•		Multi-target analysis: The most versatile module, allowing analysis of up to seven object types and flexible classification. For more information, see Guided Analysis.