 |
|
Building on experience and excellenceGE Healthcare's IN Cell Analyzer 2000 is designed to deliver the performance you need for both high content analysis and high content screening of fixed and live cells. Impressive speed, novel features that enable a wide range of applications from manual microscopy to screening and choice of standard or large camera options, set the IN Cell Analyzer 2000 apart from other lamp based cellular imagers. A configurable design allows a broad range of capabilities, from imaging of sub-cellular components to small organisms. Integrated hardware and software provides the highest quality images and data, accessible through an intuitive, and user-friendly graphical interface.
Image acquisition taken to the next level - feature highlights
|
IN Cell Analyzer 2000 builds on our experience in cellular and subcellular imaging and analysis. As such, the instrument incorporates innovative features to help you gain insights you never thought possible. Several of these image acqusition software/hardware advances are highlighted below.
|
 |
|
Rapid image acquisition
A combination of new hardware and software features gives IN Cell Analyzer 2000 significant advantages in terms of speed and suitability for higher throughput assays. • A proprietary, optimised optical path and bright 200W metal halide lamp enable imaging of a 96 well plate in
under 3 minutes for a typical 2 colour assay. This removes imaging bottlenecks and enables higher productivity
• Large chip CCD camera option enables up to 4x more information to be gathered in a single image, and capture
of a whole well when using a 2x objective
• For high throughput applications, the combination of fast laser autofocus and acquisition speed together with
a sufficient cell count function and easy robotic integration ensures a rapid turnaround
|
Manual Microscope Mode
IN Cell Analyzer 2000's Manual Microscope Mode offers a smooth transition from manual microscopy to automated, high-content analysis, leaving the researcher in complete control while significantly speeding up the workflow.
• Work with images at full-screen resolution
• Zoom in and manually adjust image
properties
• Adjust filters, objectives and z-height in real-
time before full image acquisition |
|
Manual Microscope Mode overview
|
|
|
High-performance camera options
The option of a new state-of-the art large camera (2048 x 2048 pixel array) enables superior optical zooming and captures approximately 4 times the field of view compared to the standard camera option. Coupled with a long-life wide-field illumination source that is 2 times brighter than a conventional xenon lamp, the large camera acquires high quality images that are evenly illuminated across the entire field of view. With the large camera, you can capture many more cells in a single image, giving more statistically robust results in a single pass, as well as error-free detection and quantification of large structures or rare events.
|
Preview Scan
This function, available at all magnifications, allows the user to quickly preview a selected area at any magnification before starting an acquisition run. The region of interest can be any size, from the entire plate or slide, the entire well, or a portion of a well. Zoom in and out to identify the region of interest. The imaging position can be optimised by changing the position of the field of view. The ability to select magnification, binning and exposure time for the Preview Scan gives you complete control over set-up, and means that you can acquire an informative preview image in a matter of seconds.
|
|
Preview Scan of a whole well
|
Example of Preview Scan in action: • Quickly locate the region of interest. Preview scan
quickly reveals that current field of view is over a bare
area with no cells (see panels 1 & 2 opposite) • Easily re-position the field of view to avoid artefacts
or regions of non-uniform cell density (panels 3 &
4 opposite) • Acquire image from appropriate area of the well |
|
Preview Scan in action
|
Whole well imaging
The combination of a 2x objective and large chip CCD camera enables capture of an entire well with a single image. In cases where cells are unevenly distributed or differentially affected by test treatments, collection of data from every cell in the well provides more robust statistical results. Capturing data from the entire population of a well ensures that rare events will not be missed-particularly valuable for applications such as lineage tracing, colony counting, and toxicity testing. Whole-well imaging also enables the user to image large slide tissue sections and whole organisms without the need for image stitching. Extended structures, organs, and other gross morphological features can be analyzed in their entirety without the need for stitching.
Zebra fish imaging
|
|
Stem cell colony imaging
|
Slide imaging
IN Cell Analyzer 2000 offers a simple and fast solution to acquisition of images from slides. An intuitive graphical user interface transforms slide imaging workflow, the Preview Scan mode helps you quickly locate the region of interest prior to acquisition at a higher magnification. Smart software autofocus significantly shortens run times and overlapping images can be stitched easily using IN Cell Investigator software. Slide imaging for high-content analysis is truly enabled.
|
|
|
Useful images even at low magnification
|
Imaging modes
IN Cell Analyzer 2000 includes a choice of six imaging modes (see the Modules page for details) to ensure you get the most quantitatively accurate images possible from the system for your sample type. These image restoration (deconvolution) modes are capable of removing noise, increasing contrast, and improving resolution in both the lateral (X-Y) and axial (Z) dimensions. The Z-axis projection provides a virtually extended depth-of-field, which is particularly useful when working with high magnification, high numerical aperture lenses, which provide very thin optical sections.
|
Unlike confocal approaches, which discard light emanating from above and below the focal plane, image restoration works to recover the original information, making maximum use of signal from dim samples. Image restoration is based on a realistic model of the lens system, and therefore provides more accurate results than image enhancement techniques like ‘nearest neighbor de-blurring' and ‘sharpening' provided by commercial image editing packages.
|
|
Image restoration with IN Cell Analyzer 2000
|
Transmitted light imaging
Transmitted light imaging allows label-free monitoring of cell health, movement, morphology, and growth, and can be particularly beneficial for extended time-course studies where fluorescent markers could have a toxic effect. IN Cell Analyzer 2000 simplifies the process, offering a range of modes to ensure you have the flexibility to get the highest quality images possible from your samples. |
|
The available transmitted light modes are: • Bright-field
• Differential interference contrast (DIC)
• Phase-contrast |
The high quality of transmitted light images, combined with advanced image analysis tools for segmentation, make label-free cell quantitation a reality. For added information and convenience, transmitted light images can also be analyzed in combination with images acquired in fluorescent channels in the standard IN Cell Investigator software. |
|
Combined transmitted light/fluorescent analysis
|
Image analysis and data management Transforming images into data
IN Cell Investigator combines the elements you need for analysis and interpretation into one powerful software package. Developed in collaboration with high-content users, IN Cell Investigator's multifunctional tools enable you to get started quickly - whatever your experience level with image analysis, and whatever your biological field of interest.
|
• IN Cell Analysis Modules: Ideal for the new user, a range
of fully validated, multifunctional and ready-to-use
analysis modules(includes Multi-target Analysis Module
for enhanced user definition)
• IN Cell Developer Toolbox: Perfect for more challenging
assays, a flexible tool including interactive guides to
create customized protocols
• Spotfire™ DecisionSite™: An interactive visual analysis
tool to simplify data interpretation
|
|
Images transformed into data with IN Cell Investigator
Read more about IN Cell Investigator - here >>
|
Managing the data mountain
IN Cell Miner HCM (high content management) provides advanced content management for high-content applications, allowing you to manage huge amounts of complex image, analysis, and experimental data with ease. Building on EMC Documentum™, the leading electronic content management software platform, IN Cell Miner HCM provides the performance, reliability, and scalability required to prevent bottlenecks and enable an efficient workflow. Read more here >> Outstanding image quality
|
IN Cell Analyzer 2000 delivers accurate, high speed imaging through a combination of proprietary optics, fast hardware and software autofocus, with a choice of high performance CCD cameras. This results in rapid well-to-well imaging and, combined with a bright light source, lower exposure times for increased speed with no compromise on image quality & cell health. The enhanced resolution and contrast obtained with new deconvolution features enable the acquisition of confocal-like images of exceptional quality, providing the foundation required for excellent quality high-content data.
|
|
Optical Pathway Schematic
|
Example images
Mouse intestinal tissue section
|
|
Bovine pulmonary epithelial cells
|
|
|
A modular design
A modular design means that the IN Cell Analyzer 2000 can be configured to your specification through a range of optional modules. Build the instrument that you need now, or upgrade as your needs evolve. The instrument comes with selected polychroics, filters and deconvolution software as standard. The optional modules are summarized opposite, while more details on individual modules are available on the Modules page. |
|
Optional modules include:
• Environmental control
• Liquid handling
• Transmitted light imaging
• Temperature control
• Slide imaging
• 3D deconvolution software module
• Large chip CCD camera
• Wide range of objectives - more>> |
A range of automation options For automation of high-content assays, IN Cell Analyzer 2000 has been specifically designed to be compatible with a wide range of automation options. Robots purchased from us as an option with the instrument are supported by a full integration program. Alternatively, the instrument may be interfaced with your own solutions. Read more about the automation options - here >> |
|
|
Choose your destination
