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Bia2iTC (beta release) software for integrated use of Biacore & Microcal systems

Combining kinetic & thermodynamic characterization
Kinetic characterization provides information about the rate of complex formation and its stability, whereas studies of interaction thermodynamics are required to understand why interactions occur. GE Healthcare supplies equipment for both label-free measurements of binding kinetics (Biacore™ instruments) and binding thermodynamics (MicroCal™ instruments). This provides a powerful combination of orthogonal techniques, bringing complementary information to the analysis and adding confidence to decision-making in key applications such as hit validation in drug discovery.

To facilitate the use of Biacore affinity data to optimize experimental design in complementary MicroCal assays, Bia2iTC (beta release) software is now available for free to Biacore system users (download requires a valid Biacore product key).

Please note that this beta release version is still under final development. We would therefore very much appreciate any feedback on your experiences of working with this software. Please use the web feedback form - here >>

Overview & systems supported
Affinity (K_D) values determined using Biacore are imported into this software. The program uses the K_D values to design all sample concentrations and run parameters needed to perform optimized ITC experiments. This data is subsequently exported as a setup file to the MicroCal instrument. The ITC result file is then imported by the Bia2iTC (beta release) software, enabling a comparison of the results in an Excel sheet. The combined data allows also a direct examination of possible correlations between the rate constants, affinities and the directly measured thermodynamic entities, and comparative plots can be done using Excel functionalities. The systems supported by the Bia2iTC (beta releaase) software are:

• Biacore X100 • Biacore T100 • Biacore A100 • Auto-iTC200

Bia2iTC: Integrating Biacore with MicroCal

For more details on the Bia2iTC software and its applications, please download the application note below.

Read the application note

To download a pdf of the application note, please enter your login details below. If you do not have a Biacore website account yet, you can sign up here.


			Hit validation using Biacore™ and MicroCal™analysis (619 kB)

Download and install the application

The application can be downloaded free of charge. To download, however, you need to be logged in and have a valid ‘Product Key' registered in your account details. If you do not have a Biacore website account yet, you can signup here.