His Capture Kit enables antibody capture of histidine-tagged ligands in Biacore systems
Optimized and verified assay minimizing assay development
Protocol and required solutions provided
Capture of histidine-tagged recombinant proteins is an attractive approach for protein attachment in Biacore systems for a number of reasons; i.e. physiological conditions can be used during the whole procedure. His Capture Kit is designed for capture of histidine-tagged proteins by an anti-histidine antibody and provides an alternative to capture on nickel-chelated nitrilotriacetic (NTA) groups.
Depending on the nature of the interacting molecules, this approach might give lower nonspecific binding and/or higher binding stability. The anti-histidine antibody is a monoclonal antibody (MAb) directed against polyhistidine tags in the C or N terminus of a protein.
Application example
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Kinetic characterization of papain binding to histidine-tagged cystatin B captured onto an anti-histidine antibody surface prepared with His Capture Kit.
Cystatin B and papain was kindly provided by Prof. Ingemar Björk's group at the Swedish University of Agricultural Sciences, Sweden.
Ordering information and product details
Order code: 28-9950-56
Contents: Anti-histidine antibody, immobilization buffer, and regeneration solution
Package size: Sufficient for 10 immobilization and 1000 injections
For further product details and to purchase His Capture Kit directly on line, please visit the GE Healthcare Life Sciences web catalog pages here>>
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