Simplified screening & characterization of human antibodies
Conveniently perform high quality assays with confidence
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Simplified assay development - capture format using validated antibody and other reagents
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Convenience - all reagents and pre-optimized run protocol included in kit
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Confidence - high quality reagents verified on Biacore systems
The Human Antibody Capture Kit provides the reagents and protocols required for convenient capture of human or humanized IgG antibodies onto a Biacore sensor chip surface. High quality reagents and pre-optimized protocols for immobilization of the capturing antibody and regeneration of the sensor surface facilitate rapid, reliable analyses with minimal assay development. The kit may be used with all Biacore systems except Biacore Flexchip.
About the antibody:
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Monoclonal mouse anti-human IgG (Fc) antibody
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Binds an epitope in CH2 domain of IgG Fc (all subclasses recognized)
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Little or no cross-reactivity towards other human Ig isotypes
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No cross-reactivity with IgG from other species
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Very stable binding - little or no dissociation of captured Abs
Reliable, high quality performance: Validation of specificity and capture stability of anti-human IgG (Fc) antibody
Subclass specificity
Capture stability
Application example: Characterization of therapeutic human Ab candidates: a collaboration with BioInvent AB, Sweden.
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Ranking & selection of candidates based on antigen-binding properties
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Binding assessed by kinetics and affinity
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Assay: capture of human IgG Abs, analysis of soluble antigen binding, regeneration of surface
Kinetic analysis of human Abs
Ordering information and product details
Order code: BR-1008-39
Contents: Anti-human IgG (Fc) antibody, immobilization buffer (10 mM sodium acetate pH 5.0), regeneration solution (3 M MgCl2)
Package size: Sufficient for at least 10 immobilizations and 1000 sample cycles
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