Biacore™ T200 - System Details

Outstanding sensitivity for challenging interactions
Biacore T200 enables investigations involving molecular interactions where high sensitivity is a prerequisite:
• Small organic compounds
• Low abundance molecules
• Rare or sensitive targets (e.g. GPCRs) 
• Weak interactions
• Stable complexes with slow off-rates
• Stable binders

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The microfluidics are designed to give the best possible conditions for accurate reference subtraction and are optimized for the highest quality kinetics. Biacore T200 enables measurement of kinetic constants over the broadest range, from the fastest on-rates to the slowest off-rates.

This enables confident ranking of strong binders, which can be important in antibody selection. It also enables the detection of differences among rapid binders, an important feature when studying biological processes limited by bioavailability.

Designed to support large-scale research applications
• Supports 96- and 384-well microplates
• Up to 48 hours unattended operation
• Temperature control of sample compartment
• Analyze up to 384 samples per run

Study interactions at physiological temperatures
• Analysis temperature range 4°C to 45°C
• Integrated buffer degasser ensures data quality at elevated temperatures

Rapid buffer scouting for fast assay development
• Built-in buffer selector enables up to four different buffers to be tested at one time

Sample recovery for identification by mass spectrometry
• Predefined software templates define entire recovery process
• Analytes recovered in a small volume, with minimal carry-over
• Deposition in vial containing digestion solution

High level of guidance for development and setup of assays
Software wizards and guided workflows ensure ease-of-use, and allow users to quickly get started, supporting all steps from assay development to evaluation and provides a high level of guidance for every interaction parameter.

Immobilization support for a wide range of chemistries and sensor surfaces
• Software provides predefined templates for common immobilization chemistries
• Option for customized immobilzation methods
• Chip log records important information about each immobilization and stores it with result files

Full assay design flexibility with Method Builder:
• An intuitive, powerful software that provides maximum flexibility
• Predefined methods may be used directly or modified for specific applications
• Users may also choose to develop a new, customized method

Efficient assay set-up using Data import
Biacore^TM T200 sample and position import is free-standing, add-on software that streamlines the set-up of large experiments where sample information is taken from other systems. For assay development purposes the software allows the direct import of variable assay information, e.g. variations in buffer and temperature, in one single action.

• Save precious time in assay set-up and assay development
• Seamless import of all sample information from work sheet sources
• Single step set-up of multiple step experiments

To download your free copy of Biacore T200 sample and position import software - click here.

Fast, simple kinetic analysis
Biacore T200 Software offers tools for obtaining the highest quality kinetic analysis. This can be performed using either the traditional multicycle approach (using one sample concentration per cycle), or  using single-cycle kinetics, which enables the analysis of previously difficult molecular interactions and also reduces assay development time (for more information - click here).

Biacore T200 Evaluation Software enables kinetic evaluation of interactions with a few simple clicks:
• Process multiple samples simultaneously, fit kinetic data using several
  available interaction models, with automatic subtraction of reference
   and blank data
• Automated quality control tools assess the quality of fitting in relation to
  multiple predefined parameters
• A range of tools facilitate convenient data selection, comparison and
  presentation

Efficient, reliable concentration analysis
Biacore T200 enables confident measurement of active concentration using software-supported direct binding and inhibition assays:
• Monitor sensor surface stability via repeated calibration curves, 
  providing reliable data between runs
• Ensure rigorous quality control by inclusion of control samples

Biacore T200 also offers the option of calibration-free concentration analysis (CFCA), which can be a valuable approach where no satisfactory calibration standard is available, or to check the specified concentration of a standard. To read more about CFCA - click here.

Thermodynamic analysis
Dedicated software wizards, built-in buffer degassing, and temperature control of Biacore T200 make thermodynamic analysis easier than ever.
• Obtain equilibrium thermodynamic data (values in close agreement
   with those obtained using ITC as provided by GE Healthcare
   Microcal™ systems)
• Obtain transition state thermodynamics using integration of rate
   constants measured across several temperatures to reveal the
   forces driving the interaction

Immunogenicity testing
Tools for confident detection and characterization of anti-drug antibodies (ADAs) during preclinical and clinical development are included as standard with Biacore T200.
• Minimize false negatives through confident detection of ADAs
• Detect low affinity ADAs, even in presence of drug
• Characterize ADA isotypes and binding stability
• Reduce assay development time using the assay guidelines and 
  recommendations provided

Read more about immunogenicity applications with Biacore T200
- click here