Biacore X100 is designed to enable new users to run successful assays from day one. PhD student and protein interaction analysis novice, Thuy Tran, put this theory to the test.
Research goal:
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Compare binding of several Affibody® molecules to HER2
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Determine binding specificity, affinity and on/off rates
Specificity of Affibody molecules rapidly defined
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Thuy rapidly demonstrated binding specificity for all Affibody molecules
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Compared binding levels to immobilized HER2 and a reference protein
Straightforward analysis of high affinity binders using single cycle kinetics
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Standard kinetic assays analyze a different concentration of sample in successive cycles and any remaining bound sample must be completely removed by regeneration of the surface
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Good regeneration conditions could not be found for the very high affinity variants, but Thuy used regeneration-free, single-cycle kinetics to easily and quickly acquire the data
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