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CyDye™ fluorophores are a popular choice for labeling in a variety of fluorescence based applications, such as drug-screening assays, microarrays and antibody-labelling. They are available in different chemistries to allow labeling via amine or thiol groups.

Recommendations for drug screening
For drug screening applications, we recommend the following fluorescent reagents:    
     • For fluorescence polarization and FRET - Cy3B 
     • For TRF - Cy5 in conjunction with our europium chelate, TMT.

Please see the Product Details page for further information.

Features

•   Red-shifted: compatible with LEADseeker Imaging 
    System and reduce incidence of color quenching.
•   Bright: CyDye fluors give good quantum yields and 
    have high extinction coefficients.
•   Robust: pH insensitive between 3 and 10, all CyDye
    
fluors can be used at biologically relevant pH.
•   DMSO tolerant: samples can be transferred from 
    storage to assay, without loss of performance.

•   Good aqueous solubility: can be run in biologically
    relevant assay buffers; no need for organic solvents.
•   Superior photostability compared to many other
    dyes (e.g. fluorescein).
•   Pure: QC tested to ensure high levels of
    chromophore and reactive dye content.    ´
•   Supplied with comprehensive guide to labeling
     proteins, peptides, and oligonucleotides.

Download the fluorescence screening reagents guide

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Fluorescence screening reagents guide (387 kB)