Kinase Assays

The HitHunter™ Kinase Binding Assay Platform is a unique, high-throughput screening platform to identify and characterize compounds that bind to kinase binding pockets. In contrast to activity-based assays, these assays measure ligand binding, and they are non-radioactive, homogenous, and designed for 384-well plates. They also do not require antibodies, labeled peptide substrates, or ATP.

The HitHunter Kinase Binding Assays utilize EFC technology to homogeneously measure binding of inhibitors to the ATP binding site. In the assay, the ED-inhibitor conjugate and sample compete for binding to the ATP binding site. If the conjugate is not bound to the kinase, it is free to recombine with EA (inactive EFC detection enzyme), resulting in an active ß-gal enzyme, which hydrolyzes the luminescent substrate. The signal produced by active EFC enzyme is proportional to the amount of compound bound to the kinase.

Currently, 4 different ED-inhibitor probes are available in an assay format:

ED-SB202190 for p38 MAPK
ED-Staurosporine for PKCalpha and GSK3alpha
ED-Pyrazoloprimidine for LCK
ED-PD166285 for Src and c-Kit

HitHunter kinase binding assay principle

Ordering information

	•		LCK Binding HitHunter Chemiluminescence Assay - click here
	•		p38 MAPK Binding HitHunter Chemiluminescence Assay - click here
	•		PKCα Binding HitHunter Chemiluminescence Assay - click here
	•		Src/c-Kit Binding HitHunter Chemiluminescence Assay - click here

Download product information literature

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			Kinase assays brochure_11.08.pdf (772 kB)