The kinetics of an interaction, i.e. the rates of complex formation (ka) and dissociation (kd), can be determined from the information in a sensorgram.
If binding occurs as sample passes over a prepared sensor surface, the response in the sensorgram increases. If equilibrium is reached a constant signal will be seen.
Replacing sample with buffer causes the bound molecules to dissociate and the response decreases.
Biacore evaluation software generates the values of ka and kd by fitting the data to interaction models.
The traditional approach to a kinetics assay is to inject a series of analyte concentrations over the sensor surface, one at a time, with regeneration of the surface between each analysis cycle. More recently, additional approaches have been developed to provide simpler assay development and overcome potential problems with some interaction partners, to support specific applications and to support increased throughput for kinetic assays.
• Single-cycle kinetics - more>>
• 2-over-2 kinetics - more>>